***Registration is full***
RNA-seq has emerged as a standard tool to study transcriptomes through massively parallel sequencing. The ability to generate millions of cDNA sequences makes it possible to study novel organisms as well as model organisms with unprecedented sensitivity and reproducibility. In this bootcamp, we will introduce the basic concepts of RNA-Seq and illustrate data analysis workflows through lectures and hands-on activities, including best practices for quality control, read alignment, and expression analysis. Other topics will include experimental design and basic statistical analysis of unreplicated and replicated biological sample data, alignment browsers and high performance computing techniques for dealing with transcriptomic data. Participants will spend most of their time using the Galaxy platform to work through the most common RNA-Seq data analysis workflows. Prior experience with next generation sequences, or our Next Generation Sequencing Technology Fundamentals & Applications bootcamp, is highly recommended. It is also recommended that students have some prior experience with Galaxy, or have taken our Introduction to Galaxy bootcamp.